PhanerochaeteV1, Main, Exploration, bibRecord, 000272

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.

Identifieur interne : 000272 ( Main/Exploration ); précédent : 000271; suivant : 000273

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.

Auteurs : Yoichiro Ito [Japon] ; Mamoru Yamanishi [Japon] ; Akinori Ikeuchi [Japon] ; Chie Imamura [Japon] ; Takashi Matsuyama [Japon]

Source :

PloS one [ 1932-6203 ] ; 2015.

RBID : pubmed:26692026

Descripteurs français

KwdFr :
- Cellulase (biosynthèse), Cellulase (génétique), Escherichia coli (génétique), Escherichia coli (métabolisme), Expression des gènes (MeSH), Phanerochaete (enzymologie), Phanerochaete (génétique), Protéines fongiques (biosynthèse), Protéines fongiques (génétique), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Talaromyces (enzymologie), Talaromyces (génétique).
MESH :
- biosynthèse : Cellulase, Protéines fongiques, Protéines recombinantes.
- enzymologie : Phanerochaete, Talaromyces.
- génétique : Cellulase, Escherichia coli, Phanerochaete, Protéines fongiques, Protéines recombinantes, Saccharomyces cerevisiae, Talaromyces.
- métabolisme : Escherichia coli, Saccharomyces cerevisiae.
- Expression des gènes.

English descriptors

KwdEn :
- Cellulase (biosynthesis), Cellulase (genetics), Escherichia coli (genetics), Escherichia coli (metabolism), Fungal Proteins (biosynthesis), Fungal Proteins (genetics), Gene Expression (MeSH), Phanerochaete (enzymology), Phanerochaete (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Talaromyces (enzymology), Talaromyces (genetics).
MESH :
- chemical , biosynthesis : Cellulase, Fungal Proteins, Recombinant Proteins.
- chemical , genetics : Cellulase, Fungal Proteins, Recombinant Proteins.
- enzymology : Phanerochaete, Talaromyces.
- genetics : Escherichia coli, Phanerochaete, Saccharomyces cerevisiae, Talaromyces.
- metabolism : Escherichia coli, Saccharomyces cerevisiae.
- Gene Expression.

Abstract

Combinatorial screening used together with a broad library of gene expression cassettes is expected to produce a powerful tool for the optimization of the simultaneous expression of multiple enzymes. Recently, we proposed a highly tunable protein expression system that utilized multiple genome-integrated target genes to fine-tune enzyme expression in yeast cells. This tunable system included a library of expression cassettes each composed of three gene-expression control elements that in different combinations produced a wide range of protein expression levels. In this study, four gene expression cassettes with graded protein expression levels were applied to the expression of three cellulases: cellobiohydrolase 1, cellobiohydrolase 2, and endoglucanase 2. After combinatorial screening for transgenic yeasts simultaneously secreting these three cellulases, we obtained strains with higher cellulase expressions than a strain harboring three cellulase-expression constructs within one high-performance gene expression cassette. These results show that our method will be of broad use throughout the field of metabolic engineering.

DOI: 10.1371/journal.pone.0144870
PubMed: 26692026
PubMed Central: PMC4687128

Affiliations:

Japon

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000231
to stream Main, to step Curation: 000231

Le document en format XML

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<front><div type="abstract" xml:lang="en">Combinatorial screening used together with a broad library of gene expression cassettes is expected to produce a powerful tool for the optimization of the simultaneous expression of multiple enzymes. Recently, we proposed a highly tunable protein expression system that utilized multiple genome-integrated target genes to fine-tune enzyme expression in yeast cells. This tunable system included a library of expression cassettes each composed of three gene-expression control elements that in different combinations produced a wide range of protein expression levels. In this study, four gene expression cassettes with graded protein expression levels were applied to the expression of three cellulases: cellobiohydrolase 1, cellobiohydrolase 2, and endoglucanase 2. After combinatorial screening for transgenic yeasts simultaneously secreting these three cellulases, we obtained strains with higher cellulase expressions than a strain harboring three cellulase-expression constructs within one high-performance gene expression cassette. These results show that our method will be of broad use throughout the field of metabolic engineering. </div>
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Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.

Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.

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